Obtention by Ɣ-radiation of Saccharomyces cerevisiae strains tolerant to stringent growing conditions, for bioethanol production

Authors

  • Sylvia Enid Vazquez Zeballos Laboratorio de Bioquímica y Biotecnología, Centro de Investigaciones Nucleares, Facultad de Ciencias, Uruguay
  • Luciana Buxedas Laboratorio de Bioquímica y Biotecnología, Centro de Investigaciones Nucleares, Facultad de Ciencias, Uruguay.
  • Mary Isabel Lopretti Departamento de Biotecnología y Bioprocesos, Laboratorio Tecnológico del Uruguay, Uruguay.

DOI:

https://doi.org/10.26461/07.11

Keywords:

Yeast, extremophiles, biofuels, lignin

Abstract

In order to obtain new strains of yeast capable to resist rigorous culture conditions, a fresh culture of Saccharomyces cerevisiae M522 was submitted to Ɣ-radiation. The result was a collection of strains whose capacity to tolerate elevated sugar and ethanol concentrations were tested. One of those strains was selected to analyze the effect on the culture of products of lignin degradation, the methoxylated phenolic oligomers obtained from its oxidative depolymerization by biological treatment. The enzymes involved were also studied. Spectrophotometer determinations were done at 660 nm to assess all cultures after 24 hours of incubation at 37 ˚C. It was possible to obtain the profile of the phenolic fraction by UV spectrophotometry and Laccase, Demetilase and Lig-peroxidase enzymes were identified. A strain which was called SacSV-10 was obtained with the same characteristics of the M522 in YPD. The strain was cultivated in a broth with 10 % ethanol concentration and it tolerated the effect of products of lignin degradation as well as a glucose concentration of 40 g/L, and a biomass in anaerobic conditions greater than the M522 was obtained. In conclusion, SacSV-10 is a promising candidate to be used in alcohol production from sources as lignocellulose residues.

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Published

2013-01-07

How to Cite

Vazquez Zeballos, S. E., Buxedas, L., & Lopretti, M. I. (2013). Obtention by Ɣ-radiation of Saccharomyces cerevisiae strains tolerant to stringent growing conditions, for bioethanol production. INNOTEC, (7 ene-dic), 64–68. https://doi.org/10.26461/07.11

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Articles