Autochthonous Lactococcus lactis strain: evaluation of antilisterial activity and sensory properties in Cuartirolo cheese

Authors

  • Silvana Carro Techera Departamento de Ciencia y Tecnología de la Leche, Facultad de Veterinaria, UDELAR.
  • Álvaro González Revello Departamento de Ciencia y Tecnología de la Leche, Facultad de Veterinaria, UDELAR.
  • Karina Cal Departamento de Ciencia y Tecnología de la Leche, Facultad de Veterinaria, UDELAR.
  • Sheila Giacaman Ejercicio liberal
  • Ariel Aldrovandi Departamento de Ciencia y Tecnología de la Carne, Evaluación Sensorial, Facultad de Veterinaria, UDELAR

DOI:

https://doi.org/10.26461/12.02

Keywords:

Lactic Acid Bacteria, Lactococcus lactis, Listeria monocytogenes, bacteriocin

Abstract

Acid lactic bacteria (LAB) are part of milk natural microbiota and are components of many starter cultures used in cheese making. The LAB may exhibit antimicrobial activity, based on the production of various substances, among them, peptides called bacteriocins that can be produced in situ in cheese making. Some bacteriocins include in their spectrum of action Listeria monocytogenes, pathogen associated to food that represents a risk to public health. The aim of this study was to evaluate antilisterial effect of an autochthonous LAB in Cuartirolo cheese and determine if their use altered the sensory properties of that cheese. This experimental design included two treatments: with and without autochthonous Lactococcus lactis. Cheese was divided into two groups, the first one was inoculated with Listeria innocua, making the quantification two hours, seven days and fourteen days later. Regarding sensory evaluation a descriptive sensory technique was used. In this study, Lactococcus lactis GU96743 had an antagonic activity in vitro against Listeria innocua and Listeria monocytogenes. There were also significant differences (p<0,05) between the treatments in quantification of Listeria innocua in the samples of cheese evaluated. Except for the salty flavor, the sensorial properties did not show differences between treatments.

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References

APHA, 2001. Compendium of methods for microbiological examination of foods. 4a.ed. Washington DC: APHA

Albenzio, M., Corbo, M., Rehman, S., Fox, P., De Angelis, M., Corsetti, A., Sevi, A. y Gobetti, M., 2001. Microbiological and biochemical characteristics of Canestrato Pugliese cheese made from raw milk, pasteurized milk, or by heating the curd in hot whey. En: International Journal of Food Microbiology, 67, pp.35-48.

Alegría, A., Delgado, S., Roces, C., López, B. y Mayo, B., 2010. Bacteriocins produced by wild Lactococcus lactis strains isolated from traditional, starter-free cheeses made of raw milk. En: International Journal of Food Microbiology, 143, pp.61-66.

Ananou, S., Maqueda, M., Martínez-Bueno, M., Gálvez, A. y Valdivia, E., 2005. Control of Staphylococcus aureusin sausages by enterocin AS-48. En: Meat Science, 71, pp.549–556.

Ávila, M., Garde, S., Gaya, P., Medina, M. y Nuñez, M., 2005. Influence of a bacteriocin-producing lactic culture on proteolysis and texture of Hispánico cheese. En: International Dairy Journal, 15, pp.145-153.

Ayad, E.H.E., Nashat, S., El-Sadek, N., Metwaly, H. y El-Soda, M., 2004. Selection of wild lactic acid bacteria isolated from traditional Egyptian dairy products according to production and technological criteria. En: Food Microbiology, 21, pp.715-725.

Balciunas, E. M., Martinez, F. A. C., Todorov, S. D., de Melo Franco, B. D. G., Converti, A., & de Souza Oliveira, R. P., 2013. Novel biotechnological applications of bacteriocins: a review. En: Food Control, 32, pp.134-142.

Barba, J.L. y Piard, J.C., 1993. Plantaricins S and T, two new bacteriocins produced by Lactobacillus plantarum LPCO10 isolated from a green olive fermentation. En: Applied and Environmental Microbiology, 59, pp.1416-1422.

Bérodier, F., Lavanchy, P., Zannoni, M., Casals, J., Herrero, L. y Adamo, C., 1997. Guide d’Évaluation Olfacto-Gustative des Fromages à Pâte Dure et Semi-dure. En: Lebensmittel-Wissenschaft und Technologie, 30, pp.653-664.

Bromberg, R., Moreno, I., Delboni, R.R., Cintra, H.C. y Oliveira, P.T.C., 2005. Characteristics of the bacteriocin produced by Lactococcus lactis subsp. Cremoris CTC 204 and the effect of this compound on the mesophilic bacteria associated with raw beef. En: World Journal of Microbiology & Biotechnology, 21, pp.351-358.

Cosentino, S., Fadda, M., Deplano, M., Melis, R., Pomata, R. y Pisano, M.B., 2012. Antilisterial activity of nisin-like bacteriocin-producing Lactococcus lactis subsp. lactis isolated from traditional Sardinian dairy products. En: Journal of Biomedicine and Biotechnology, ID 376428. doi:10.1155/2012/376428

Cruz-Chamorro, L.; Puertollano, M.A.; Puertollano, E.; Álvarez de Cienfuegos, G.; de Pablo, M. 2006. In vitro biological activities of magainin alone or in combination with nisin. En: Peptides 27 (6):1201-1209.

Chen, H. y Hoover, D.G., 2003. Bacteriocins and their food applications. En: Comprehensive Reviews in Food Science and Food Safety, 2, pp.82-100.

Dal Bello, B., Cocolin, L., Zeppa, G., Field, D., Cotter, P. y Hill, C., 2012. Technological characterization of bacteriocin producing Lactococcus lactis strains employed to control Listeria monocytogenes in Cottage cheese. En: International Journal of Food Microbiology, 153, pp.58-65.

Devlieghere, F., Vermeiren, L. y Debevere, J., 2004. New preservation technologies and limitations. En: International Dairy Journal, 14, pp.273-285.

De Vuyst, L. y Leroy, F., 2007. Bacteriocins from lactic acid bacteria: production, purification, and food applications. En: Journal of Molecular Microbiology and Biotechnology, 13, pp.194–199.

De Vuyst, L. y Vandamme, E., 1994. Bacteriocins of lactic acid bacteria: microbiology, genetics and application. London: Chapman & Hall, Ltd.

Fraga, M., Perelmuter, K., Delucchi, L., Cidade, E. y Zunino, P., 2008. Vaginal lactic acid bacteria in the mare: evaluation of the probiotic potential of native Lactobacillus spp. and Enterococcus spp. strains. En: Antonie van Leeuwenhoek, 93, pp.71-78.

Fraga, M., Perelmuter, K., Giachaman, S., Zunino, P. y Carro, S., 2013. Antimicrobial properties of lactic acid bacteria isolated from Uruguayan artisan cheese. En: Food Science and Technology, 33, pp.801-804.

Fraga, M., Scavone, P. y Zunino, P., 2005. Preventive and therapeutic administration of an indigenous Lactobacillussp. strain against Proteus mirabilis ascending urinary tract infection in a mouse model. En: Antonie van Leeuwenhoek,88, pp.25-34.

Gandhi, M. y Chikindas, M.L., 2007. Listeria: A foodborne pathogen that knows how to survive. En: International Journal of Food Microbiology, 113, pp.1-15.

Garde, M., Ávila, M., Medina, M. y Núñez, M., 2005. Influence of a bacteriocin producing lactic culture on the volatile compounds, odour and aroma of Hispánico cheese. En: International Dairy Journal,15, pp.1034-1043.

González, B., Arca, P., Mayo, B. y Suarez, J.E., 1994. Detection, Purification, and Partial Characterization of plantaricin C, a bacteriocin produces by a Lactobacillus plantarumstrain of dairy origin. En: Applied and Environmental Microbiology, 60, pp.2158-2163.

Ghrairi, T., Frere, J., Berjeaud, J.M. y Manai, M., 2004. Lactococcin mMT24, a novel two-peptide bacteriocin produced by Lactococcus lactis isolated from rigouta cheese. En: International Journal of Food Microbiology, 105, pp.389–398.

Hammer, Ø., Harper, D.A. y Ryan, P.D., 2001. PAST: Paleontological statistics software package for education and data analysis. En: Paleontología Electrónica, 4, pp.1-9.

Hartmann, H., Wilke, T. y Erdmann, R., 2011. Efficacy of bacteriocin-containing cellfree culture supernatants from lactic acid bacteria to control Listeria monocytogenes in food. En: International Journal of Food Microbiology, 146, pp.192-199.

International Dairy Federation, 1990. Milk and milk products detection of Listeria monocytogenes. Provisional recommended method 143. Bruselas: IDF.

International Organization for Standardization ISO 22935- 1:2009 (IDF 99-1:2009). Milk and milk products -- Sensory analysis -- Part 1: General guidance for the recruitment, selection, training and monitoring of assessors. Génova: ISO.

Joerger, M. y Klaenhammer, T., 1986. Characterization and purification of helveticin J and evidence for a chromosomally determined bacteriocin produced by Lactobacillus helveticus 481. En: Journal of Bacteriology, 167, pp.439-446.

Juncioni de Arauz, L., Faustino Jozala, A., Gava Mazzola, P. y Vessoni Penna, T., 2009. Nisin biotechnological production and application: a review. En: Trends in Food Science & Technology, 20, pp.146-154.

Lavanchy, P., Berodier, F., Zannoni, M., Noël, Y., Adamo, C., Squella, J. y Herrero, L., 1993. L’Evaluation Sensorielle de la Texture des Fromages à Pâte Dure ou Semi-Dure. Etude Interlaboratoires. En: Lebensmittel-Wissenschaft und Technologie, 26, pp.59-68.

Lundén, J., Tolvanen, R. y Korkeala, H., 2004. Human Listeriosis Outbreaks Linked to Dairy Products in Europe. En: Journal of Dairy Science, 87, pp.E6–E11.

Lü, X., Hu P., Dang, Y. y Liu, B., 2014. Purification and partial characterization of a novel bacteriocin produced by Lactobacillus casei TN-2 isolated from fermented camel milk (Shubat) of Xinjiang Uygur Autonomous region, China. En: Food Control, 43, pp.276-283.

Maisnier-Patin, S.; Deschamps, N.; Tatini, S.R.; Richard, J., 1992. Inhibition of Listeria monocytogenes in Camembert cheese made with a nisin-producing starter. En: Lait, 72, pp.249-263.

Ministerio de Salud Pública (MSP), 1994. Reglamento Bromatológico Nacional. Decreto Nº 315/994 2ª ed.Montevideo: IMPO, CD ROM.

Patin, S., Deschamps, N., Tatini, S.R. y Richard, J., 1992. Inhibition of Listeria monocytogenes in Camembert cheese made with a nisin-producing starter. En: Lait, 72, pp.249-263.
Martínez, B., 1996. Bacteriocinas de Lactococcus lactis aislados de quesos asturianos: nisina z y lactococina 972. Asturias: Universidad de Oviedo. (Tesis doctoral)

Mathot, A., Beliard, E. y Thuault, D., 2003. Streptococcus thermophilus 580 Produces a Bacteriocin Potentially Suitable for Inhibition of Clostridium tyrobutyricum in Hard Cheese. En: Journal of Dairy Science, 86, pp.3068-3074.

Mendonça, P., Martins, L., Tassinari, M., Keizo, A., Nogueira, G. y Nero, L., 2010. Protocols for the isolation and detection of lactic acid bacteria with bacteriocinogenic potencial. En: LWT-Food Science and Technology, 43, pp.1320-1324.

Montero, H., Aranibar, G., Cañameras, C. y Castañeda, R., 2005. Metodología para la caracterización de quesos argentinos. En: INTI-Lácteos. En: JASLIS 2005, Argentina (6-8 de septiembre de 2005). Buenos Aires: [s.n.].

Montville, T.J y Chen, Y., 1998. Mechanistic action of pediocin and nisin: recent progress and unresolved question. En: Applied Microbiology and Biotechnology, 50(5), pp.511-9

Oppegård, C., Fimland, G., Thorbæk, L. y Nissen-Meyer, J., 2007. Analysis of the Two-Peptide Bacteriocins Lactococcin g and Enterocin 1071 by Site-Directed Mutagenesis. En: Applied and Environmental Microbiology,73, pp.2931-2938.

Ortolani, M.B.T., Moraes, P.M., Perin, L.M., Viçosa, G.N., Carvalho, K.G., Silva Junior, A y Nero, L.A., 2010. Molecular identification of naturally occurring bacteriocinogenic and bacteriocinogenic-like lactic acid bacteria in raw milk and soft cheese. En: Journal of Dairy Science, 93, pp.2880-2886.

Osuntoki, A.A., Gbenle, G.O. y Olukoya, D.K., 2003. Evidence for chromosomal determination of fungicidal activity in strain of Lactobacillus brevis and Lactobacillus fermentum isolated from fermented foods. En: Folia Microbiologica,48, pp.56-58.

Pinto, M., Vega y León, M. y Pérez, N., 1998. Métodos de análisis de la leche y derivados. Valdivia: Universidad Austral de Chile.

Randazzo, C., Caggia, C. y Neviani, E., 2009. Application of molecular approaches to study lactic acid bacteria in artisanal cheeses. En: Journal of Microbiology Methods, 78, pp.1-9.

Rodríguez, E., Calzada, J., Arqués, J.L., Rodríguez, J.M., Nuñez, M. y Medina, M., 2005. Antimicrobial activity of pediocin-producing Lactococcus lactis on Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157:H7 in cheese. En: International Dairy Journal, 15, pp.51-57.

Ryser, E.T., 1999. Incidence and behavior of Listeria monocytogenes in cheese and other fermented dairy products. En: Ryser, E.T. y Marth, E.H. (eds.). Listeria, listeriosis and food safety. New York: Marcel Dekker. pp.411-503.

Samelis, J., Kakouri, A., Rogga, K., Savvaidis, I. y Kontominas, M., 2003. Nisin treatments to control Listeria monocytogenespost-processing contamination on Anthotyros, a traditional Greek whey cheese, stored at 4°C in vacuum packages. En: Food Microbiology, 20, pp.661-669.

Silva, P., Pereira, D., Oliveira, L. y Costa Junior, L., 1997. Físico-Química do leite e derivados. Métodos analíticos. Minas Gerais: Oficina de Impressão Gráfica e Editora Ltda. Juiz de Fora.

Stead, S.L., Ashwin, H., Richmond, S.F., Sharman, M., Langeveld, P.C., Barendse, J.P., Stark, J. y Keely, B.J., 2008. Evaluation and validation according to international standards of the Delvotest® SP-NT screening assay for antimicrobial drugs in milk. En: International Dairy Journal, 18, pp.3-11.

Stiles, M.E., 1993. Bacteriocins produced by Leuconostoc species. En: Journal of Dairy Science, 77, pp.2718-2724.

Sulzer, G. y Busse, M., 1991. Growth inhibition of Listeria spp. on Camembert cheese by bacteria producing inhibitory substances. En: International Journal of Food Microbiology, 14, pp.287-296.

Taheri, P., Samadi, N., Ehsani, M.R., Khoshayand, M.R. y Jamalifar, H., 2012. An evaluation and partial characterization of a bacteriocin produced by Lactococcus lactis subsp lactis ST1 isolated from goat milk. En: Brazilian Journal of Microbiology, 43, pp.1452-1462.

Todorov, S.D. y Dicks, L.M.T., 2005. Lactobacillus plantarumisolates from molasses produces bacteriocins active against Gram-Negative bacteria. En: Enzyme and Microbial Te c h n o l o g y, 36, pp.318-326.

Todorov, S.D. y Dicks, L.M., 2005b. Optimization of bacteriocin ST311LD production by Enterococcus faecium ST311LD, isolated from spoiled black olives. En: Journal of Microbiology, 43, pp.370-374.

Todorov, S.D., Nyati, H., Meincken, M. y Dicks, L.M., 2007. Partial characterization of bacteriocin AMA-K, produced by Lactobacillus plantarum AMA-K isolated from naturally fermented milk from Zimbabwe. En: Food Control, 18, pp.656-664.

Turner, D., Lamosa, P., Rodríguez, A. y Martínez, B., 2013. Structure and properties of the metastable bacteriocin Lcn972 from Lactococcus lactis. En: Journal of Molecular Structure, 1031, pp.207–210

Trmcic, A., Obermajer, T., Rogelj, I. y Bogovic Matijasic, B., 2008. Short communication: Culture-independent detection of lactic acid bacteria bacteriocin genes in two traditional Slovenian raw milk cheeses and their microbial consortia. En: Journal of Dairy Science, 91, pp.4535-4541.

Valbuena, E., Barreiro, J., Sánchez, E., Castro, G., Kutchinskaya, V. Y Briñez, W., 2008. Predicción del crecimiento de Lactococcus lactis subsp. lactis en leche descremada estéril en función a la temperatura. En: Revista Científica Facultad Ciencias Veterinarias- Universidad de Zulia- Venezuela FCV-LUZ, 18, pp.745-758.

Venema, K., Venema, G. y Kok, J., 1995. Lactococcal bacteriocins: mode of action and immunity. En: Trends in Microbiology, 3, pp.299-304.

Weiss, A. y Hammes, W., 2005 Lactic acid bacteria as protective cultures against Listeria spp. on cold-smoked salmon. En: European Food Research and Technology, 222, pp.343-346.

Wouters, J.T., Ayad, E.H., Hugenholtz, J. y Smit, G., 2002. Microbes from raw milk for fermented dairy products. En: International Dairy Journal, 12, pp.91-109.

Published

2016-12-14

How to Cite

Carro Techera, S., González Revello, Álvaro, Cal, K., Giacaman, S., & Aldrovandi, A. (2016). Autochthonous Lactococcus lactis strain: evaluation of antilisterial activity and sensory properties in Cuartirolo cheese. INNOTEC, (12 ago-dic), 15–26. https://doi.org/10.26461/12.02

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