Comparación del tamaño de nanopartículas de oro empleando diferentes técnicas y protocolos de medición

Santiago Botasini

doi:10.26461/21.02

Autores

Santiago Botasini Laboratorio de Biomateriales, Instituto de Química Biológica, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay https://orcid.org/0000-0002-6488-5886

DOI:

https://doi.org/10.26461/21.02

Palavras-chave:

nanopartículas, ouro, tamanho, colóides, nanometrologia

Resumo

Com o crescente avanço da nanotecnologia, tanto na esfera acadêmica quanto comercial, aumentou a necessidade de técnicas e protocolos de caracterização de nanopartículas. Embora existam vários métodos de medição, o tamanho "verdadeiro" das nanopartículas não pode ser entendido em termos absolutos, mas está relacionado ao tipo de material de cada técnica e aos protocolos ou critérios associados a essas medidas. Em outras palavras, quando falamos sobre o tamanho de uma partícula, devemos ter em mente duas perguntas básicas: o que estamos medindo? e como medimos? Do ponto de vista da metrologia, é importante focar a discussão nos critérios a serem levados em consideração e quais parâmetros adicionais devemos reportar ao apresentar um resultado de tamanho de nanopartícula. O presente trabalho mostra, para fins comparativos, a caracterização do tamanho de nanopartículas homogêneas de ouro, utilizando diferentes técnicas e critérios de medição para os métodos rotineiros de DLS, UV-VIS e HR-TEM. Os resultados mostram como, quando falamos em tamanho de partículas, é necessário fazer referência ao modelo utilizado, bem como aos critérios escolhidos ao fazer a contagem.

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Referências

Amendola, V. y Meneghetti, M., 2009. Size evaluation of gold nanoparticles by UV-vis spectroscopy. En:Journal of Physical Chemistry C,113(11), pp.4277–4285. https://doi.org/10.1021/jp8082425.

Bienert, R., Emmerling, F. y Thünemann, A.F., 2009. The size distribution of “gold standard” nanoparticles. En: Analytical and Bioanalytical Chemistry,395(6), pp.1651–1660.doi: https://doi.org/10.1007/s00216-009-3049-5 .

Brito-Silva, A.M., Sobral-Filho, R.G., Barbosa-Silva, R., de Araújo, C.B., Galembeck,A. y Brolo, A.G., 2013. Improved synthesis of gold and silver nanoshells. En: Langmuir,29(13), pp.4366–4372. https://doi.org/10.1021/la3050626 .

Bohren, C. y Huffman, D., 2004. Absorption and scattering of light by small particles. Derby: Wiley.

Domingos, R.F., Baalousha, M., Ju-Nam, Y., Reid, M., Tufenkji, N., Lead, J., Leppard, G. y Wilkinson, K., 2009. Characterizing manufactured nanoparticles in the environment: multimethod determination of particle sizes. En: Environ Sci Technol,43, pp.7277-7284. https://doi.org/10.1021/es900249m .

Eaton, P. Quaresma, P., Soares, C., Neves, C., de Almeida, M.P., Pereira, E. y West, P., 2017. A direct comparison of experimental methods to measure dimensions of synthetic nanoparticles. En: Ultramicroscopy,182, pp.179–190. https://doi.org/10.1016/j.ultramic.2017.07.001

Fissan, H., Ristig, S., Kaminski, H., Asbacha, C. y Epplebc, M., 2014. Comparison of different characterization methods for nanoparticle dispersions before and after aerosolization. En: Analytical Methods,6(18), pp.7324–7334. doi: https://doi.org/10.1039/c4ay01203h .

International Organization for Standarization, 2017. ISO 22412: Particle size analysis — Dynamic light scattering (DLS).Ginebra: ISO.

Hinterwirth, H., Wiedmer, S., Moilanen, M., Lehner, A., Allmaier, G., Waitz, T., Lindner, W. y Lämmerhofer, M., 2013. Comparative method evaluation for size and size-distribution analysis of gold nanoparticles. En: Journal of Separation Science,36(17), pp.2952–2961. doi: https://doi.org/10.1002/jssc.201300460 .

Khlebtsov, B.N. y Khlebtsov, N.G., 2011. On the measurement of gold nanoparticle sizes by the dynamic light scattering method. En: Colloid Journal,73(1), pp.118–127. doi: https://doi.org/10.1134/S1061933X11010078 .

Laven, P., 2018. MiePlot[En linea]. [s.l.]: [s.n]. [Consulta: 18 de mayo de 2020]. Disponible en:http://www.philiplaven.com/mieplot.htm .

Liu, X., Atwater, M., Wang, J. y Huo, Q., 2007. Extinction coefficient of gold nanoparticles with different sizes and different capping ligands. En: Colloids and Surfaces B: Biointerfaces,58(1), pp.3–7.[Consulta: 18 de mayo de 2020]. Disponible en: https://doi.org/10.1016/j.colsurfb.2006.08.005 .

Meli, F., Klein, T., Buhr, E., Frase, C., Gleber, G., Krumrey, M., Duta, A., Duta, S., Korpelainen, V., Bellotti, R., Picotto, G., Boyd, R. y Cuenat, A., 2012. Traceable size determination of nanoparticles, a comparison among European metrology institutes. En: Measurement Science and Technology,23(12). doi:https://doi.org/10.1088/0957-0233/23/12/125005 .

Méndez, Eduardo y Botasini, Santiago, 2019. Synthesis of ultra-homogeneous gold nanoparticles. En: Proceedings of the World Congress on New Technologies,(NewTech’19). Lisboa: International ASET. pp. 11159. DOI: https://doi.org/10.11159/icnfa19.152.

Minelli, C., Bartczak, D., Peters, R., Rissler, J., Undas, A., Sikora, A., Sjöström, E., Goenaga-Infante, H. y Shard, A., 2019. Sticky measurement problem: number concentration of agglomerated nanoparticles. En: Langmuir, 35(14), pp.4927–4935. https://doi.org/10.1021/acs.langmuir.8b04209.

National Institutes of Health y Laboratory for Optical and Computational Instrumentation, 2019. ImageJ [En linea]. Versión 1-8-0_112. Bethesda: NIH. [Consulta: 18 de mayo de 2020]. Disponible en: https://imagej.nih.gov/ij/download.html.

Nelson, B.C., Atha, D., Elliott, J., Marquis, B., Petersen, E., Cleveland, D., Watson, S., Tseng, I., Dillon, A., Theodore, M. y Jackman, J., 2013. NIST gold nanoparticle reference materials do not induce oxidative DNA damage. En:Nanotoxicology,7(1), pp.21–29. DOI:https://doi.org/10.3109/17435390.2011.626537.

OriginLab, 2020. Origin Lab [En linea]. Northampton: Originlab. [Consulta: 18 de mayo de 2020]. Disponible en:https://www.originlab.com/.

Rasmussen, K., Rauscher, H., Mech, A., Riego Sintes, J., Gillialand, D., Gonzales, M., Kearns, P., Moss, K., Visser, M., Groenewold, M. y Bleeker, E.A.J., 2018. Physico-chemical properties of manufactured nanomaterials - Characterisation and relevant methods. An outlook based on the OECD Testing Programme. En: Regulatory Toxicology and Pharmacology, 92, pp.8-28. https://doi.org/10.1016/j.yrtph.2017.10.019 .

Rice, S.B., Chan, C., Brown, S.C., Eschbach, P., Han, L., Ensor, D.S., Stefaniak, A.B., Bonevich, J., Vladár, A. E., Hight Walker, A.R., Zheng, J., Starnes, C., Stromberg, A., Ye, J. y Grulke, E.A., 2013. Particle size distributions by transmission electron microscopy: an interlaboratory comparison case study. En: Metrologia, 50(6), pp.663–678. https://doi.org/10.1088/0026-1394/50/6/663 .

Rogers, K.R., Navratilova, J., Stefaniak, A., Bowers, L., Knepp, A., Al-Abed, S., Potter, P., Gitipour, A., Radwan, I., Nelson, C. y Bradham, K., 2018. Characterization of engineered nanoparticles in commercially available spray disinfectant products advertised to contain colloidal silver. En:Science of the Total Environment, 619–620, pp.1375–1384. https://doi.org/10.1016/j.scitotenv.2017.11.195 .

Segelstein, D., 1981.The complex refractive index of water.Kansas City: University of Missouri.

Shard, A.G., Wright, L. y Minelli, C., 2018. Robust and accurate measurements of gold nanoparticle concentrations using UV-visible spectrophotometry. En:Biointerphases,13(6), p.061002. https://doi.org/10.1116/1.5054780.

Soliwoda, K., Rosowski, Marcin, Tomaszewska, Emilia, Tkacz-Szczesna, Beata, Celichowski, Grzegorz, Psarski, Maciej y Grobelny, Jaroslaw, 2015. Synthesis of monodisperse gold nanoparticles via electrospray-assisted chemical reduction method in cyclohexane. En: Colloids and Surfaces A: Physicochemical and Engineering Aspects, 482, pp.148–153. https://doi.org/10.1016/j.colsurfa.2015.04.040 .

Souza, T.G.F., Ciminelli, V.S.T. y Mohallem, N.D.S., 2016. A comparison of TEM and DLS methods to characterize size distribution of ceramic nanoparticles. En: Journal of Physics: Conference Series, 733(1). doi: https://doi.org/10.1088/1742-6596/733/1/012039 .

Tanaka, L.S., 2019. Regulación blanda, normas técnicas y armonización regulatoria internacional, para la nanotecnología. En: Mundo Nano, 13(24), pp.1-27. https://doi.org/10.22201/ceiich.24485691e.2020.24.69621 .

Turkevich, J., 1985. Colloidal gold. Part II. En:Gold Bull, 18, pp.125–131. doi: https://doi.org/10.1007/BF03214694.