Avaliar a eficácia do subproduto de levedura de cerveja da indústria cervejeira como bioestimulante em hidroponia

Autores

  • María Magdalena Vazquez Instituto de Investigaciones en Producción, Sanidad y Ambiente (IIPROSAM), Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Mar del Plata, Mar del Plata, Argentina. https://orcid.org/0000-0001-8765-5322
  • Silvina Quintana Instituto de Investigaciones en Producción, Sanidad y Ambiente (IIPROSAM), Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Mar del Plata, Mar del Plata, Argentina. https://orcid.org/0000-0003-1845-7677
  • Sandra Medici Instituto de Investigaciones en Producción, Sanidad y Ambiente (IIPROSAM), Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Mar del Plata, Mar del Plata, Argentina. https://orcid.org/0000-0002-2465-2068
  • Liesel Brenda Gende Instituto de Investigaciones en Producción, Sanidad y Ambiente (IIPROSAM), Consejo Nacional de Investigaciones Científicas y Técnicas - Universidad Nacional de Mar del Plata, Mar del Plata, Argentina. https://orcid.org/0000-0002-8056-3510

DOI:

https://doi.org/10.26461/24.05

Palavras-chave:

PGPM, sustentabilidade, Arabidopsis thaliana, Eruca vesicaria, Lactuca sativa

Resumo

A capacidade de alguns microrganismos de estimular o crescimento e desenvolvimento de plantas é conhecida. No entanto, o uso de levedura residual da indústria cervejeira artesanal como agente promotor de crescimento vegetal (PGPM) tem sido brevemente estudado. O objetivo deste trabalho foi caracterizar e analisar o uso de levedura residual da indústria cervejeira em hortaliças hidropônicas. Nós avaliamos os efeitos da adição de levedura no crescimento de plantas de Eruca vesicaria, Arabidopsis thaliana e Lactuca sativa com esta técnica. Um aumento significativo no comprimento da raiz principal e no número de raízes laterais foi observado após o tratamento com as suspensões de levedura em A. thaliana e E. vesicaria cultivadas em mudas (SN 4.28 ± 0.15, S05 7.30 ± 0.29; SN 39.68 ± 2.20, S05 57.37 ± 2.80, respectivamente). Em sistemas hidropônicos de raízes flutuantes, a inoculação de leveduras à solução hidropônica aumentou a área radicular de L. sativa em 30% em relação ao controle. Nossos resultados confirmam que a estirpe J14 de Saccharomyces cerevisiae da indústria cervejeira pode prover benefícios em cultivos hidropônicos com efeitos positivos no crescimento radicular. Esses resultados validam a potencial aplicação da cepa J14 de S. cerevisiae no cultivo de plantas em hidroponia como ferramenta biotecnológica.

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Referências

Abdul Ameer, M. A. y Hussein, H. F., 2020. Induction of Rhizophagy by yeast Saccharomyces cerevisiae in roots of lettuce Lactuca sativa roots of lettuce Lactuca sativa. En: J. Phys.: Conf. Ser., 1664(1), 012116. DOI: http://dx.doi.org/10.1088/1742-6596/1664/1/012116

Abhilash, P.C., Dubey R.K., Tripathi, V., Gupta, V.K. y Singh, H.B., 2016. Plant growth-promoting microorganisms for environmental sustainability. En: Trends Biotechnol, 34(11), pp.847–850. DOI: https://doi.org/10.1016/j.tibtech.2016.05.005

Abhilash, P.C., Tripathi, V., Edrisi, S.A. Gupta, V.K. y Singh, H.B., 2019. Sustainability of crop production from polluted lands. En: Energ. Ecol. Environ., 1, pp.54-65. DOI: https://doi.org/10.1007/s40974-016-0007-x

Amprayn, K., Rose, M. T., Kecskés, M., Pereg, L., Thanh, H. y Kennedy, I.R., 2012. Plant growth promoting characteristics of soil yeast (Candida tropicalis HY) and its effectiveness for promoting rice growth. En: Appl. Soil Ecol., 61, pp.295-299. DOI: https://doi.org/10.1016/j.apsoil.2011.11.009

AOAC International, 1984. Official Methods of Analysis of AOAC International. 14a ed. Washington: AOAC. Official Method 2.062.

AOAC International, 1998. Official Methods of Analysis of AOAC International. 15a ed. Washington: AOAC. Official Method 942.05.

Baras, T., 2018. DIY hydroponic gardens: how to design and build an inexpensive system for growing plants in water. Beverly: Cool Springs Press. ISBN-10: 0760357595.

Bedolla-Torres, M. H., Palacios, A., Palacios, O. A., Choix, F. J., Jesús F. De, Valle A., López, D.R., Luis, J., Villavicencio, E., Luna, R. De, Trujillob, A. G., Avila Serranod, N. Y. y Ortega Pérez, R., 2015. La irrigación con levaduras incrementa el contenido nutricional del forraje verde hidropónico de maíz. En: Rev Argent Microbiol., 47(3), pp. 236-244. DOI: https://doi.org/10.1016/j.ram.2015.04.002

Bekuma, A., 2019. Nutritional benefit and economic value of hydroponics fodder production technology in sustainable livestock production against climate change – a mini-review. En: Adv. Appl. Sci., 4(1), pp.23-25.

Bewley, J. D. y Black M. eds., 1982. Physiology and biochemistry of seeds in relation to germination. Vol. 1. Development, germination and growth. Berlin: Springer Verlag. ISBN: 978-3-642-68643-6.

Boratyn, M. G., Camacho, C., Cooper, P. S., Coulouris, G., Fong, A., Ma, N., Madden, T. L., Matten, W.T., McGinnis, S. D., Merezhuk, Y., Raytselis, Y., Sayers, R.W., Tao, Ye J. y Zaretskaya, I., 2013. BLAST: a more efficient report with usability improvements. En: Nucleic Acids Research, 41(1), pp. 29–33, DOI: https://doi.org/10.1093/nar/gkt282

Cloete, K. J., Valentine, A. J., Stander, M. A., Blomerus, L. M. y Botha, A., 2009. Evidence of symbiosis between the soil yeast Cryptococcus laurentii and a sclerophyllous medicinal shrub, Agathosma betulina (Berg.) Pillans. En: Microb Ecol., 57(4), pp. 624-32. DOI: https://doi.org/10.1007/s00248-008-9457-9

Compant, S., Samad, A., Faist, H. y Sessitsch, A. A review on the plant microbiome: Ecology, functions and emerging trends in microbial applications. En: J. Adv., 19, pp. 29–37. DOI: https://doi.org/10.1016/j.jare.2019.03.004

El-Tarabily, K. A. y Sivasithamparam, K., 2006. Potential of yeasts as biocontrol agents of soil-borne fungal plant pathogens and as plant growth promoters. En: Mycoscience, 47, pp. 25–35. DOI: https://doi.10.1007/s10267-005-0268-2

Falih, A. M. y Wainwright, M., 1995. Nitrification, S-oxidation and P-solubilization by the soil yeast Williopsis californica and by Saccharomyces cerevisiae. En: Mycol Res, 99(2), pp. 200-204. DOI: https://doi.org/10.1016/S0953-7562(09)80886-1

Freimoser, F. M., Paula, M., Mejia, R., Tilocca, B. y Migheli, Q., 2019. Biocontrol yeasts: mechanisms and applications. En: World J Microbiol Biotechnol, 35(10), pp.154. DOI: https://doi.org/10.1007/s11274-019-2728-4

Glick, B. R., 2012. Plant growth-promoting bacteria: mechanisms and applications. En: Scientifica, ID 963401. DOI: https://doi.org/10.6064/2012/963401

Gómez Alonso, S., Gutiérrez, I. H. y García-Romero, E., 2007. Simultaneus HPLC Analysis of Biogenic Amines, amino acids and ammonium ion as aminoenone derivates in wine and beer samples. En: J. Agric. Food Chem., 55(3), pp. 608-613. DOI: https://doi.org/10.1021/jf062820m

GraphPad, 2007. Prism. Vers. 1992-2007 5.01. San Diego: GraphPad Sofware, Inc.

Hardoim, P. R., van Overbeek, L. S., Berg, G., Pirttilä, A. M., Compant, S. y Campisano, A. 2015. The hidden world within plants: ecological and evolutionary considerations for defining functioning of microbial endophytes. En: Microbiol Mol Biol Rev, 79(3), pp. 293-320. DOI: https://doi.org/10.1128%2FMMBR.00050-14

Joshi, M., Vaishnava, C. S. y Sharma, S. K., 2018. Economical analysis of feeding hydroponics maize fodder with and without supplementation of probiotic (Saccharomyces Cerevisae) in gir calves. En: Int. J. Sci. Environ. Technol, 7(3), pp. 809-814. ISSN 2278-3687

Kurtzman, C. P. y Robnett, C. J., 1997. Identification of clinically important ascomycetous yeasts based on nucleotide divergence in the 59 end of the large-subunit (26S) ribosomal DNA gene. En: J. Clin. Microbiol, 35(5), pp. 1216–1223. DOI: https://doi.org/10.1128/jcm.35.5.1216-1223.1997

Lonhienne, T., Mason, M. G., Raganm M. A., Hugenholtz, P., Schmidt y S., Paungfoo-lonhienne, C., 2015. Yeast as a biofertilizer alters plant growth and morphology. En: Crop Sci., 54(2), pp.785–790. DOI: https://doi.org/10.2135/cropsci2013.07.0488

Malamy, J. E. y Benfey, P.N., 1997. Organization and cell differentiation in lateral roots of Arabidopsis thaliana. En: Development, 124(1), pp. 33–44. DOI: https://doi.org/10.1242/dev.124.1.33

Martínez-Mate, M. A., Martin-Gorriz, B., Martínez-Álvarez, V., Soto-García, M., Maestre-Valero, J. F. y 2018. Hydroponic system and desalinated seawater as an alternative farm-productive proposal in water scarcity areas: energy and greenhouse gas emissions analysis of lettuce production in southeast Spain. En: J Clean Prod, 172, pp.1298–310. DOI: https://doi.org/10.1016/j.jclepro.2017.10.275

Marulanda, C. e Izquierdo, J., 2003. La huerta hidropónica popular [En línea]. Santiago: FAO. [Consulta: 1 de noviembre 2022]. Disponible en: https://www.fao.org/3/ah501s/ah501s.pdf

Mukherjee, A., Verma, J. P. y Hesham, A. E., 2020. Yeast a potential bio-agent: future for plant growth and postharvest disease management for sustainable agriculture. En: Appl Microbiol Biotechnol Yeast, 104(4), pp. 1497-1510. DOI: https://doi.org/10.1007/s00253-019-10321-3

Naamala, J. y Smith, D. L., 2020. Relevance of plant growth promoting microorganisms and their derived compounds, in the face of climate change. En: Agronomy, 10(8), pp. 1179. DOI: https://doi.org/10.3390/agronomy10081179

Nassar, A., El-Tarabily, K. A. y Sivasithamparam, K., 2005. Promotion of plant growth by an auxin-producing isolate of the yeast Williopsis saturnus endophytic in maize (Zea mays L.) roots. En: Biol Fertil Soils, 42, pp. 97–108. DOI: https://doi.org/10.1007/s00374-005-0008-y

Nutaratat, P., Srisuk, N., Arunrattiyakorn, P. y Limtong, S., 2014. Plant growth-promoting traits of epiphytic and endophytic yeasts isolated from rice and sugar cane leaves in Thailand. En: Fungal Biol, 118(8), pp. 683–94. DOI: https://doi.org/10.1016/j.funbio.2014.04.010

Olajire, A. A., 2012. The brewing industry and environmental challenges. En: J Clean Prod, 256, 102817. DOI: https://doi.org/10.1016/j.jclepro.2012.03.003

Paradiso, R., Arena, C., Miccom V. De, Giordano, M., Aronne, G. y Pascale, S. De., 2017. Changes in leaf anatomical traits enhanced photosynthetic activity of soybean grown in hydroponics with plant growth-promoting. En: Front. Plant Sci, 8, 674. DOI: http://doi: 10.3389/fpls.2017.00674

Philippot, L., Raaijmakers, J. M., Lemanceau, P., Putten, W. H. V. D., 2013. Going back to the roots: the microbial ecology of the rhizosphere. En: Rev. Microbiol., 11, pp. 789–799. DOI: https://doi.org/10.1038/nrmicro3109

Ramayo Cruz, P., 2018. Aprovechamiento de subproductos derivados de la elaboración de cerveza artesanal. Trabajo presentado para optar al título del máster universitario en gestión de calidad y trazabilidad en alimentos de origen vegetal [En línea]. Badajoz: Universidad de Extremadura. [Consulta: 1 de marzo de 2022]. Disponible en: https://dehesa.unex.es/handle/10662/8081

Resh, M. H., 2015. Hydroponics for the home grower. Boca Ratón: CRC Press. DOI: https://doi.org/10.1201/b18069

Rincón Reyna, J. F., Rincón Reyna, P. G., Torres Maravilla, E., Mondragón Rojas, A. G., Sánchez Pardo, M. E., Arana Cuenca, A., Ortiz Moreno, A., Jiménez García, E., 2016. Caracterización fisicoquímica y funcional de la fibra de mesocarpio de coco (Cocos nucifera L.) [En línea]. En: Investigación y Desarrollo en Ciencia y Tecnología de Alimentos, 1(2), pp. 279-284. [Consulta: 1 de noviembre de 2022]. Disponible en: http://www.fcb.uanl.mx/IDCyTA/files/volume1/2/3/49.pdf

Sambo, P., Nicoletto, C., Giro, A., Pii, Y., Valentinuzzi, F., Mimmo, T., Lugli, P., Orzes, G., Mazzetto, F., Astolfi, S., Terzano, R. y Cesco, S., 2019. Hydroponic solutions for soilless production systems: issues and opportunities in a smart agriculture perspective. En: Front. Plant Sci., 10, pp.923. DOI: https://doi.org/10.3389/fpls.2019.00923

Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch S., Rueden C., Saalfeld S., Schmid B, Tinevez J-Y, White DJ., Hartenstein V, Eliceiri K., Tomancak P. y Cardona, A., 2012. Fiji: an open-source platform for biological-image analysis. En: Nat Methods, 9, pp. 676–682. DOI: https://doi.org/10.1038/nmeth.2019

Smart, K., Chambers, M., Lambert, I., Jenkins, C. y Smart, C.A., 1999. Use of methylene violet staining procedures to determine yeast viability and vitality. En: J Am Soc Brew Chem, 57(1), pp. 18-23. DOI: https://doi.org/10.1094/ASBCJ-57-0018

Villanueva, N. S., 2021. Mecanismos de inducción de rizobios para reducir el estrés por sequía en las leguminosas. En: Revista de Investigaciones Altoandinas, 23(4), pp. 258-265. DOI: https://dx.doi.org/10.18271/ria.2021.263

Weigel, D. y Glazebrook, J., eds., 2002. Arabidopsis: a laboratory manual. New York: Cold Spring Harbor Laboratory Press. ISBN-10: 0879695730.

Yam, K. L. y Papadakis, S. E., 2004. A simple digital imaging method for measuring and analyzing color of food surfaces. En: J. Food Process Eng., 61, pp.137–142. DOI: https://doi.org/10.1016/S0260-8774(03)00195-X

Publicado

2022-12-20

Como Citar

Vazquez, M. M., Quintana, S. ., Medici, S. ., & Gende , L. B. . (2022). Avaliar a eficácia do subproduto de levedura de cerveja da indústria cervejeira como bioestimulante em hidroponia. INNOTEC, (24 jul-dic), e622. https://doi.org/10.26461/24.05

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