Sensibilidad a antibióticos, actividad antibacteriana frente a patógenos y fermentaciones ácido lácticas de una cepa nativa de Lactobacillus paracasei de origen humano

Authors

  • Fabiana Rey Departamento de Biotecnología y Bioproceso de, Laboratorio Tecnológico del Uruguay (LATU), Uruguay
  • Sylvia Vázuez Departamento de Biotecnología y Bioproceso de, Laboratorio Tecnológico del Uruguay (LATU), Laboratorio de Bioquímica y Biotecnología, Centro de Investigación Nucleares, Facultad de Ciencias, Universidad de la República, Uruguay
  • Mary Lopretti Departamento de Biotecnología y Bioproceso de, Laboratorio Tecnológico del Uruguay, Laboratorio de Bioquímica y Biotecnología, Centro de Investigación Nucleares, Facultad de Ciencias, Universidad de la República, Uruguay
  • Pablo Zunino Departamento de Mricobiología, Instituto de Investigaciones Biológica Clemente Estable, Uruguay

DOI:

https://doi.org/10.26461/05.06

Abstract

The objective of this study was the characterization of a native Lactobacillus paracasei strain isolated at the Bioprocesses and Biotechnology Department of the Technological Laboratory of Uruguay (LATU). Lactic acid fermentations were made in order to determinate the strain growth’s characteristics. We evaluated three kinetics: the organic acids by titration in milk, acidification of the medium throughout the fermentation by pH measurement and biomass produced in 10 ml of culture medium. The anti-microbial activity against Salmonella sp. a gastrointestinal pathogen, was tested by means of a disk diffusion test. Sensitivity to penicillin and dicloxacillin was assayed through a modified antibiogram. The strain showed good growth in milk, with growth curves characteristic of lactic bacteria. Halos of growth inhibition of Salmonella sp. were detected depending on the bacterial concentration. Curves were obtained for inhibition of bacterial growth as a function of concentrations of antibiotic. In conclusion, this strain of Lactobacillus paracasei isolated from human faeces is a promising candidate to be used in the formulation of probiotic products.

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References

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How to Cite

Rey, F., Vázuez, S., Lopretti, M., & Zunino, P. (2011). Sensibilidad a antibióticos, actividad antibacteriana frente a patógenos y fermentaciones ácido lácticas de una cepa nativa de Lactobacillus paracasei de origen humano. INNOTEC, (5 ene-dic), 39–42. https://doi.org/10.26461/05.06

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